Coding

Part:BBa_K2556001:Design

Designed by: Yankang Wu   Group: iGEM18_ZJUT-China   (2018-10-04)


Cas9 from Streptococcus pyogenes


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3378
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cas9 gene sequence contains the EcoRI restriction site, so we adapted it to RFC10 by codon optimization.


Source

Streptococcus pyogenes

References